Conclusion : in this paper , we show huccr5 n - terminal gene fragment was cloned successfully and its specific antibody was prpared . by these we not only introduced a simple and quick method to get a specific antibody f ( ab ' ) 2of certain functional domain but also a good idea and technique to study other high similar superfamily members 中有效表达ccrsn端膜外第一谬基因片段，并制备出抗hllccrsn端的特异性抗体f （ ah ’ ） 。 。本方法为一简捷快速的特定功能结构域抗体厂（ ah ’ ） 。
Objective : to investigate the prevalence of hepatitis b virus genotype in fujian province and the clinical implications of distinct genotypes in hbv - related liver disease ; and three different methods were compared in monitoring the lamivudine - resistant hbv mutants to find a more sensitive , special and quick method which can be used method to study the correlation between hbv genotypes and lamivudine therapy in the treatment of chronic hepatitis b . methods : 1 目的：首次调查福建省hbv基因型的分布状况以及hbv基因型与hbv相关肝病临床的可能相关性；同时对临床上常用的三种检测ymdd突变株的方法进行比较，探寻一种能够准确、敏感、快速有效地检测出ymdd突变株的实验方法，为研究hbv基因型与拉米夫定治疗慢乙肝的疗效提供可靠的方法学。方法： 1
